UJI ANTIBAKTERI EKSTRAK KOMBINASI DAUN PEGAGAN (CENTELLA ASIATICA) DAN SURUHAN (PEPEROMIA PELLUCIDA L. KUNTH) TERHADAP BAKTERI PROPIONIBACTERIUM ACNES
Abstract
Propionibacterium acnes is one of the normal skin flora that can cause acne. Acne treatments can be done by utilizing natural ingredients. Pegagan and suruhan leaves contain flavonoids, alkaloids, saponins, tannins, and triterpenoids that have antibacterial activity. This study aims to determine the antibacterial activity of the combined extract of pegagan and suruhan leaves against Propionibacterium acnes. This research is a pre-experimental research with a shot case study design using the Kirby-Bauer disc diffusion method. There were four concentrations of the combined extracts that were tested, there were 20, 40, 60, and 80%, as well as 30 µg chloramphenicol and 96% ethanol as positive and negative controls. The results showed that the combined extracts of pegagan and suruhan leaves were able to inhibit the growth of Propionibacterium acnes with the inhibition zone diameters of 4.41; 6.91; 7.25; and 7.91 mm at concentrations of 20, 40, 60, and 80%, respectively. The zone of inhibition of Propionibacterium acnes growth was proportional to the concentration of the combination extracts. Kolmogorov Smirnov test showed a value of ρ>α (0.05) which proved that the data was normally distributed. One Way Anova test showed the value of ρ<α (0.05) which proves that there are differences in the inhibition zone at various concentrations. Based on these results, it can be concluded that the combined extract of pegagan and suruhan leaves has antibacterial activity against the growth of Propionibacterium acnes.
References
[2] Karnirius Harefa, Barita Aritonang, and Ahmad Hafizullah Ritonga, “Aktivitas Antibakteri Ekstrak Etanol Kulit Markisa Ungu (Passiflora Edulis Sims) Terhadap Bakteri Propionibacterium Acnes,” J. Multidisiplin Madani, vol. 2, no. 6, pp. 2743–2758, 2022, doi: 10.55927/mudima.v2i6.469.
[3] Z. Z. Khan, M. Assi, and T. A. Moore, “Recurrent Epidural Abscess Caused by Propionibacterium Acnes,” Kansas J. Med., vol. 2, no. 4, pp. 92–95, 2009, doi: 10.17161/kjm.v2i4.11302.
[4] K. E. K. Dewi, N. Habibah, and N. Mastra, “Uji Daya Hambat Berbagai Konsentrasi Perasan Jeruk Lemon Terhadap Bakteri Propionibacterium acnes,” JST (Jurnal Sains dan Teknol., vol. 9, no. 1, pp. 86–93, 2020, doi: 10.23887/jstundiksha.v9i1.19216.
[5] A. N. W. Pratama, M. H. Pradipta, and A. Machlaurin, “Survei Pengetahuan dan Pilihan Pengobatan Jerawat diKalangan Mahasiswa Kesehatan Universitas Jember (A Survey on Knowledge and Treatment Options of Acne Vulgaris Among Health Science Students of Universitas Jember),” J. Pustaka Kesehat., vol. 5, no. 2, pp. 389–393, 2017.
[6] R. N. Afriyanti, “Artikel Review Akne Vulgaris Pada Remaja,” J. Major., vol. 4, no. 6, pp. 102–109, 2015.
[7] C. Dessinioti and A. Katsambas, “Antibiotics and Antimicrobial Resistance in Acne: Epidemiological Trends and Clinical Practice Considerations,” Yale J. Biol. Med., vol. 95, no. 4, pp. 429–433, 2022.
[8] N. L. B. Artaningsih, N. Habibah, and M. Nyoman, “Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans secara In-Vitro,” J. Kesehat., vol. 9, no. 3, p. 336, 2018, doi: 10.26630/jk.v9i3.967.
[9] L. P. I. V. Vinenthy, N. Habibah, and I. G. A. S. Dhyanaputri, “Uji Daya Hambat Perasan Bawang Putih terhadap Pertumbuhan Salmonella typhi,” J. Kesehat., vol. 10, no. 3, p. 354, 2019, doi: 10.26630/jk.v10i3.1547.
[10] I. G. A. . Dharmawati, N. Habibah, I. G. A. A. . Swastini, and H. S. Bekti, “Antibacterial Potential of Spondias pinnata (L.f) kurz Leaf Ethanol Extract against Streptococcus mutans Bacterial Growth,” Biomed. Pharmacol. J., vol. 15, no. 3, pp. 1647–1651, 2022, doi: 10.13005/bpj/2502.
[11] H. S. Bekti, I. G. A. A. Dharmawati, and N. Habibah, “Uji Ekstrak Daun Cemcem Dalam Menghambat Pertumbuhan Bakteri Phorphyromonas Gingivalis,” JST (Jurnal Sains dan Teknol., vol. 11, no. 2, pp. 267–273, 2022, doi: 10.23887/jstundiksha.v11i2.49457.
[12] N. Habibah, H. S. W. Nugroho, I. G. A. S. Dhyanaputri, I. G. A. A. Dharmawati, and I. B. O. Suyasa, “Phytochemical profile and bioactive compounds of beluntas leaves extract (Pluchea Indica L.) and its hydrogel preparations,” J. Med. Pharm. Allied Sci., vol. 12, no. 6, pp. 6184–6190, 2023, doi: 10.55522/jmpas.V12I6.5672.
[13] K. D. P. Niluwih, I. G. A. A. P. Swastini, N. Habibah, and I. B. O. Suyasa, “The Ethanol Extract Of Jukut Pendul (Kyllinga Nemoralis) Exhibits Inhibitory Activity Against The Growth Of Staphylococcus Aureus,” Meditory J. Med. Lab., vol. 11, no. 2, pp. 122–132, 2023, doi: 10.33992/meditory.v11i2.3060.
[14] A. Siregar, M. S. Mutia, and A. Napiah, “Uji Aktivitas Antibakteri Ekstrak Etanol Daun Pegagan (Centella asiatica (L.) Urb ) pada Bakteri Staphylococcus aureus,” Pharm. J. Islam. Pharm., vol. 6, no. 1, pp. 21–28, 2022, doi: 10.21111/pharmasipha.v6i1.7403.
[15] D. Purwaningsih and D. Wulandari, “Uji Aktivitas Antibakteri Ekstrak Etanol Daun Suruhan (Peperomia pellucida L. Kunth) Terhadap Bakteri Pseudomonas aeruginosa ATCC 27853,” Biota J. Ilm. Ilmu-Ilmu Hayati, vol. 5, no. 1, pp. 1–7, 2020, doi: 10.24002/biota.v5i1.3077.
[16] F. Azzahra and M. Hayati, “Uji Aktivitas Ekstrak Daun Pegagan (Centella Asiatica (L). Urb) Terhadap Pertumbuhan Streptococcus mutans,” B-Dent J. Kedokt. Gigi Univ. Baiturrahmah, vol. 5, no. 1, pp. 9–19, 2019, doi: 10.33854/jbd.v5i1.133.
[17] F. Salahudin and H. A. Cahyanto, “Aktivitas Antibakteri Propionibacterium Acnes Dan Formulasi Ekstrak Etanol Biji Pinang (Areca Catechu, L) Dalam Krim Anti Jerawat,” J. Ris. Ind. Has. Hutan, vol. 12, no. 1, p. 21, 2020, doi: 10.24111/jrihh.v12i1.5424.
[18] D. R. Ningsih, P. Purwati, Z. Zusfahair, and A. Nurdin, “Hand Sanitizer Ekstrak Metanol Daun Mangga Arumanis (Mangifera indica L.),” Alchemy J. Penelit. Kim., vol. 15, no. 1, pp. 10–23, 2019, doi: 10.20961/alchemy.15.1.21458.10-23.
[19] S. Atun, “Metode Isolasi dan Identifikasi Struktural Senyawa Organik Bahan Alam,” J. Konserv. Cagar Budaya Borobudur, vol. 8, no. 2, pp. 53–61, 2014, doi: 10.33374/jurnalkonservasicagarbudaya.v8i2.132.
[20] R. D. Rahmitasari, D. Suryani, and N. I. Hanifa, “Aktivitas Antibakteri Ekstrak Etanolik Daun Juwet (Syzygium cumini (L.) Skeels) terhadap Bakteri Isolat Klinis Salmonella typhi,” Pharm. J. Farm. Indones. (Pharmaceutical J. Indones., vol. 17, no. 1, p. 138, 2020, doi: 10.30595/pharmacy.v17i1.6448.
[21] E. Kusumawati, A. Apriliana, and R. Yulia, “Kemampuan Antibakteri Ekstrak Etanol Daun Nangka (Atrocarpus heterophyllus Lam.) Terhadap Escherichia coli,” J. Sains dan Kesehat., vol. 1, no. 7, pp. 327–332, 2017, doi: 10.25026/jsk.v1i7.51.
[22] R. Hasyati and D. Meilani, “Uji Aktivitas Antibakteri Gram Positif Kombinasi Ekstrak Etanol Daun Pegagan ( Centella asiatica ( L .) Urb .) Dan Daun Bandotan ( Ageratum conyzoides L .),” FARMASAINKES J. Farm. Sains, dan Kesehat., vol. 1, no. 2, pp. 168–174, 2022.
[23] S. Sudrajat, S. Sadani, and S. Sudiastuti, “Analisis Fitokimia Senyawa Metabolit Sekunder Ekstrak Kasar Etanol Daun Meranti Merah (Shorea leprosula Miq.) dan Sifat Antibakterinya terhadap Staphylococcus aureus dan Eschericia coli,” J. Trop. Pharm. Chem., vol. 1, no. 4, pp. 303–311, 2012, doi: 10.25026/jtpc.v1i4.41.
[24] N. Habibah, G. Ayu, and M. Ratih, “Antibacterial Activity of Pineapple Infused Arak Bali Against Escherichia coli,” in Proceeding International Conference on Health Polytechnic Ministry of Health Surabaya, 2023, pp. 1–12
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