UJI ANTIOKSIDAN EKSTRAK PIGMEN KAROTENOID DAN SITRULIN PADA KULIT BUAH BLEWAH (Cucumis melo L.) SECARA IN VITRO (METODE DPPH)

  • Antonius Padua Ratu Sekolah Tinggi Teknologi Industri dan Farmasi Bogor
  • Nadia Fahmi Silabi PT Saraswanti Indo Genetech, Bogor
  • Padmono Citroreksoko Sekolah Tinggi Teknologi Industri dan Farmasi Bogor
Keywords: Antioxidant, Carotene, Citrulline, Cucumis melo L

Abstract

Antioxidants stabilize free radicals with a complete the lack of electrons from free radicals and inhibiting the chain reaction of free radical formation. The purpose this research is to determine the amount of the antioxidant potential of the cantaloupe skin extracts and the effect of different test result of pigment carotene and citrulline in skin cantaloupe extracts to the extraction stage. Cantaloupe skin with drying and without drying treatment is gradually extracted using three solvents (petroleum ether-acetone-water). The highest antioxidant activity shown by extracts water without drying treatment (667.30 mg / L), which was significantly different (P <0.05) with acetone and petroleum ether (996.65 and 2368.26 mg / L). Treatment with drying only on petroleum ether extract (1607.28 mg / L), which provides activity compared with acetone and water (2871.54 and 2290.75 mg / L). However, cantaloupe skin extract antioxidant activity was lower compared with β-carotene and vitamin C. Treatment without drying also give the highest pigments carotenoid shown by extracts aceton (β-caroten 11,32 mg/L, lycopene 0,29 mg/100g) and level of citrulline in water extracts (29,91 mg/L). The difference of effect in simplisia treatment and solvent on the activity of antioxidants and pigments carotenoid levels were significantly different (P <0.05), whereas the levels of citrulline in the simplisia treatment was not significantly different for solvent acetone and significantly different (P <0.05) for solvent petroleum ether and water.

References

[1]. Kumalaningsih. 2007. Antioksidan dan Penangkal Radikal Bebas. Jakarta: Trubus Agrisarana.
[2]. Windono, T., S. Soediman, U. Yudawati, E. Ermawati, A. Srielita, and T.I. Erowati. 2001. Uji Peredam Radikal Bebas terhadap 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) dari Ekstrak Kulit Buah dan Biji Anggur (Vitis vinifera L.) Probolinggo Biru dan Bali. Artocarpus. 1:34-43.
[3]. Fiedor, J., and K. Burda. 2014. Potential Role of Carotenoids as Antioxidant in Human Health and Disease. Nutrients 6: 466-488.
[4]. Khomsan, A., dan Y. Harlinawati. 2008. Terapi Jus untuk Reumatik dan Asam Urat. Jakarta: Niaga Swadaya
[5]. Bohm, V. 2002. Trolox Equivalent Antioxidant Capacity of Different Geometrical Isomer of α-Carotene, β-Carotene, Lycopene, and Zeaxanthin. J. Agric. Food Chem. 50: 221-226
[6]. Fuhrman, B., A. Elis, and M. Aviram. 1997. Hypocholesterolemic Effect of Lycopene and β-Carotene is Related to Suppression of Cholesterol Synthesis and Augmentation of LDL Receptor Activity in Macrophages. Biochem Biophys Res Commun. 233(3):658–662.
[7]. Akashi, K., C. Miyake, and A. Yakota. 2001. Citrulline, a Novel Compatible Solute in Drought-Tolerant Wild Watermelon Leaves, is an Efficient Hydroxyl Radical Scavanger. FEBS Letters. 508: 438-442.
[8]. Departemen Kesehatan Republik Indonesia. Farmakope Herbal Indonesia Edisi I. Jakarta ; 2008, h 169.
[9]. Harborne, J.B. 1998. Phytochemical Methods. UK: Chapman and Hall.
[10]. Octaviani, T., A. Guntarti, dan H. Susanti. 2014. Penetapan Kadar β-Karoten Pada Beberapa Jenis Cabe (Genus Capsicum) Dengan Metode Spektrofotometri Tampak. Pharmaciana. 4 (2): 101-109.
[11]. Alda, L.M., I. Gogoasa, and D.M. Bordean. 2009. Lycopene content of tomoatoes and
tomato product. J Agroalomentary Process and Technologies. 15 (4): 540-542.
[12]. Davis, A.R., W. Fish, A. Levi, S. King, T. Wehner, and P. Perkins-Veazie, P. 2010. L-Citrulline Levels in Watermelon Cultivars From Three Locations. Cucurbit Genet. Coop. Rpt. 33:36–39.
[13]. King, S.R., W. Liu, S. Zhao, Z. Cheng, X. Wan, and Z. Yan. 2010. Lycopene and Sitrulin Contents in Watermelon (Citrullus lanatus) Fruit with Different Ploidy and Changes during Fruit Development. ISHS Acta Horticulturae. 871: 543-547.
[14]. Disoschi, A.M. 2009. Total Antioxidant Capacity of Some Commercial Fruit Juice. Electrochemical And Spectrophotometrical Approaches.Molecules 14: 480-493.
[15]. Jomova, K., O. Kysel , J.C. Madden, H. Morris, S.J. Enoch, S. Budzak, A.J. Young, M.T.D. Cronin, M. Mazur, M. Valko. 2009. Electron transfer from all-trans β-carotene to the t-butyl peroxyl radical at low oxygen pressure (an EPR spectroscopy and computational study). Chemical Physics Letters 478: 266–270.
[16]. Othmer, Kirk. 2004. Kirk-Othmer Encyclopedia of Chemical Technology. 5th ed. Volumes 1: New York: John Wiley and Sons.
[17]. Lide, D.R. 2005. CRC Handbook of Chemistry and Physics. 86th Edition. Boca Raton : CRC Press, p. 3-88
[18]. [BASF]. 2005. β-Carotene. German: BASF
[19]. Curis, E., I. Nicolis, C. Moinard, S. Osowska, N. Zerrouk, S. Benazeth, and L. Cynober. 2005. Almost all about sitrulin in mammals. Amino Acids 29: 177-205
Published
2016-06-13
How to Cite
Antonius Padua Ratu, Nadia Fahmi Silabi, & Padmono Citroreksoko. (2016). UJI ANTIOKSIDAN EKSTRAK PIGMEN KAROTENOID DAN SITRULIN PADA KULIT BUAH BLEWAH (Cucumis melo L.) SECARA IN VITRO (METODE DPPH). Jurnal Farmamedika (Pharmamedika Journal), 1(1), 1-11. https://doi.org/10.47219/ath.v1i1.39