PENGARUH EKSTRAK MORINGA OLEIFERA TERHADAP AKTIVITAS ANTIOKSIDAN SEDIAAN SERUM
DOI:
https://doi.org/10.47219/ath.v10i1.461Keywords:
kelor, antioksidan, spektrofotometer uv vis, dpph, moringa oliefera, serum, sediaan, ekstrakAbstract
Kelor (Moringa oleifera) dikenal sebagai "miracle plant" karena kandungan senyawa antioksidannya yang sangat tinggi, menjadikannya sumber antioksidan alami yang potensial dan bermanfaat bagi tubuh. Salah satu pemanfaatannya adalah dengan mengekstrak daun kelor dan mengolahnya menjadi sediaan serum yang dapat melindungi kulit dari kerusakan akibat stres oksidatif. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun kelor terhadap aktivitas antioksidan dalam sediaan serum. Ekstrak daun kelor diperoleh menggunakan metode maserasi yang kemudian diformulasikan dalam sediaan serum. Untuk mengukur aktivitas antioksidan sedian serum, digunakannya metode 1,1-diphenyl-2-picrylhydrazyl (DPPH) dengan spektrofotometer UV-Vis sehingga diperoleh nilai IC50. Hasil penelitian menunjukkan nilai IC50 sediaan serum dengan ekstrak daun kelor sebanyak 0%, 3%, dan 6% secara berturut-turut 19467.42 ?g/ml, 11910.89 ?g/ml, dan 7889.45 ?g/ml. Dengan demikian, terdapat pengaruh penambahan ekstrak daun kelor pada formula sediaan serum. Aktivitas antioksidan akan meningkat seiring dengan bertambahnya konsentrasi ekstrak daun kelor yang digunakan. Namun, untuk mencapai efektivitas antioksidan yang maksimal, diperlukan metode ekstraksi dan formulasi serum yang lebih tepat dan efisien.
References
[2] M. Hasanuzzaman et al., “Regulation of Reactive Oxygen Species and Antioxidant Defense in Plants under Salinity,” Int. J. Mol. Sci., vol. 22, no. 17, p. 9326, Aug. 2021, doi: 10.3390/ijms22179326.
[3] A. V. Snezhkina et al., “ROS Generation and Antioxidant Defense Systems in Normal and Malignant Cells,” Oxid. Med. Cell. Longev., vol. 2019, pp. 1–17, Aug. 2019, doi: 10.1155/2019/6175804.
[4] A. Yadav, R. Kumari, A. Yadav, J. P. Mishra, S. Srivatva, and S. Prabha, “Antioxidants and its functions in human body - A Review,” Res Env. Life Sci, vol. 9, no. 11, pp. 1328–1331, 2016.
[5] L. He, T. He, S. Farrar, L. Ji, T. Liu, and X. Ma, “Antioxidants Maintain Cellular Redox Homeostasis by Elimination of Reactive Oxygen Species,” Cell. Physiol. Biochem., vol. 44, no. 2, pp. 532–553, 2017, doi: 10.1159/000485089.
[6] Akbarirad, H, G. A. A, K. M S., and M. K. A, “An overview on some of important sources of natural antioxidants,” Int. Food Res. J., vol. 23, no. 3, pp. 928–933, 2016.
[7] E. A. Shalaby, S. M. M. Shanab, W. M. A. El-Raheem, and E. A. Hanafy, “Biological activities and antioxidant potential of different biosynthesized nanoparticles of Moringa oleifera,” Sci. Rep., vol. 12, no. 1, p. 18400, Nov. 2022, doi: 10.1038/s41598-022-23164-2.
[8] E. Chukwuebuka, “Moringa oleifera ‘The Mother’s Best Friend,’” Int. J. Nutr. Food Sci., vol. 4, no. 6, p. 624, 2015, doi: 10.11648/j.ijnfs.20150406.14.
[9] A. R. Abdulkadir, D. D. binti Zawawi, A. K. Yunusa, and M. S. Jahan, “In Vitro Anti-Oxidant Potential, Total Phenolic Content and Total Flavonoid Content of Methanolic Flower and Seed Extract of Miracle Tree (Moringa Oleifera Lam.),” Aust. J. Basic Appl. Sci., vol. 9, no. 20, pp. 27–31, 2015.
[10] C. Suphachai, “Antioxidant and anticancer activities of Moringa oleifera leaves,” J. Med. Plants Res., vol. 8, no. 7, pp. 318–325, Feb. 2014, doi: 10.5897/JMPR2013.5353.
[11] O. V. Volnyanska, M. O. Mironyak, V. D. Myrhorodska-Terentieva, T. N. Avdienko, M. V. Nikolenko, and Ukrainian State University of Science and Technologies, “Development of a cosmetic product for the face based on hyaluronic acid and honey,” Vopr. Khimii Khimicheskoi Tekhnologii, no. 4, pp. 17–24, Sep. 2024, doi: 10.32434/0321-4095-2024-155-4-17-24.
[12] N. Khan, S. Ahmed, M. A. Sheraz, Z. Anwar, and I. Ahmad, “Pharmaceutical based cosmetic serums,” in Profiles of Drug Substances, Excipients and Related Methodology, vol. 48, Elsevier, 2023, pp. 167–210. doi: 10.1016/bs.podrm.2022.11.006.
[13] R. Hanifah, S. Sukmawati, and N. Amalia, “Uji aktivitas antioksidan sediaan serum wajah ekstrak etanol daun kelor (moringa oleifera lam.) dengan metode DPPH,” J. Pharmacopolium, vol. 6, no. 2, Jan. 2024, doi: 10.36465/jop.v6i2.1204.
[14] B. L. Manurung, E. Monica, and R. Rollando, “Formulasi dan evaluasi antioksidan daun kelor Moringa oleifer L dalam sediaan serum dengan metode senyawa radikal DPPH,” Sainsbertek J. Ilm. Sains Teknol., vol. 3, no. 2, pp. 66–77, Mar. 2023, doi: 10.33479/sb.v3i2.233.
[15] M. G. Rasul, “Conventional Extraction Methods Use in Medicinal Plants, their Advantages and Disadvantages,” 2019. [Online]. Available: https://api.semanticscholar.org/CorpusID:245903807
[16] ?. Gulcin and S. H. Alwasel, “DPPH Radical Scavenging Assay,” Processes, vol. 11, no. 8, p. 2248, Jul. 2023, doi: 10.3390/pr11082248.
[17] Y. D. Mardhiani, “Formulasi dan stabilitas sediaan serum dari ekstrak kopi hijau (coffea canephora var. Robusta) sebagai antioksidan,” Indones. Nat. Res. Pharm. J. INRPJ, vol. 2, no. 2, pp. 19–33, 2018.
[18] S. Chatterjee, N. Goswami, and P. Bhatnagar, “Estimation of Phenolic Components and in vitro Antioxidant Activity of Fennel (Foeniculum vulgare) and Ajwain (Trachyspermum ammi) seeds,” Adv. Bioresearch, vol. 3, no. 2, pp. 109–118, 2012.
[19] M. Ezz El-Din Ibrahim, R. M. Alqurashi, and F. Y. Alfaraj, “Antioxidant Activity of Moringa oleifera and Olive Olea europaea L. Leaf Powders and Extracts on Quality and Oxidation Stability of Chicken Burgers,” Antioxidants, vol. 11, no. 3, p. 496, Mar. 2022, doi: 10.3390/antiox11030496.
[20] S. Kumar, R. Sandhir, and S. Ojha, “Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves,” BMC Res. Notes, vol. 7, no. 1, p. 560, Dec. 2014, doi: 10.1186/1756-0500-7-560.
[21] R. ElGamal, C. Song, A. M. Rayan, C. Liu, S. Al-Rejaie, and G. ElMasry, “Thermal Degradation of Bioactive Compounds during Drying Process of Horticultural and Agronomic Products: A Comprehensive Overview,” Agronomy, vol. 13, no. 6, p. 1580, Jun. 2023, doi: 10.3390/agronomy13061580.
[22] W. N. F. Azra, B. Susilo, N. Izza, Abd. Rohim, and R. P. Samudra, “Extraction of Bioactive Compounds in Moringa Leaves ( Moringa oleifera Lam. ) using Modified Sonication Technique,” BIO Web Conf., vol. 123, p. 02003, 2024, doi: 10.1051/bioconf/202412302003.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Putri Rose Amanda Puri, Suparno
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ciptaan disebarluaskan di bawah 