AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL 70% DAN INFUSA DAUN KECIPIR (Psophocarpus tetragonolobus (L.) DC.) DENGAN METODE PERENDAMAN RADIKAL BEBAS

  • Eem Masaenah Sekolah Tinggi Teknologi Industri dan Farmasi Bogor
  • Anna Priangani Roswiem Sekolah Tinggi Teknologi Industri dan Farmasi Bogor
  • Devina Putri Sekolah Tinggi Teknologi Industri dan Farmasi Bogor
Keywords: Antioxidant, DPPH, Extract, Infusion, Psophocarpus tetragonolobus (L.) DC

Abstract

Winged leaf (Psophocarpus tetragonolobus (L.) DC.) has been known to contain vitamin C compounds which can be efficacious as antioxidants. The aims of this study was determine the secondary metabolites contained in 70% ethanol extract and winged leaf (Psophocarpus tetragonolobus (L.) DC) infusion and to test the antioxidant activity of 70% ethanol extract and winged leaf infusion (Psophocarpus tetragonolobus (L.) DC). ) with the free radical reduction method using DPPH. Ethanol extract of 70% winged leaf (Psophocarpus tetragonolobus (L.) DC.) was made with a series of concentrations of 10, 15, 20, 25, 30 ppm and infusion of winged leaf (Psophocarpus tetragonolobus (L.) DC) with a series of concentrations of 75, 100, 125, 150, 175 ppm are both dissolved with methanol pro analysis and DPPH, then their absorbance is measured using a UV-Vis spectrophotometer at a wavelength of 517 nm. Ethanol extract of 70% winged leaf (Psophocarpus tetragonolobus (L.) DC.) Contains flavonoid, saponin, tannin, steroids and winged leaf infusion (Psophocarpus tetragonolobus (L.) DC) contains flavonoid, saponin, tannin. Ethanol extract of 70% winged leaf (Psophocarpus tetragonolobus (L.) DC.) Has antioxidant activity with IC50 value of 22.12 ppm (very strong) and winged leaf infusion (Psophocarpus tetragonolobus (L.) DC.) of 167.03 ppm (weak) and vitamin C as a positive control of 6.82 ppm (very strong).

References

[1]. Ahmad,A.R., Mun’im A., dan Elya B. 2012. Study of antioxidant activity with reduction of DPPH radical and xanthin oxidase inhibitor of the extract of Ruellia Tuberosa Linn Leaf . International Research Journal Fo Pharmacy: 66.
[2]. Da’i M., Wulandari M.M., Utami W. 2011. Uji Aktivitas Penangkap Radikal DPPH Oleh Analog Kurkumin Monoketon Dan N-Heteroalifatik Monoketon. Surakarta: Universitas Muhammadiyah Surakarta, Vol. 12 No. 1: 19-25.
[3]. Handayani T. 2013. KECIPIR, Potensi Lokal Yang Terpinggirkan. Bandung : IPTEK Tanaman Sayuran, No. 001: 2.
[4]. Safitri V. 2018. Uji Aktivitas Antioksidan Fase n-Hexan, Etil Asetat, dan Air Dari Ekstrak Etanol 96% Daun Kecipir (Psophocarpus tetragonolobus (L.) [Skripsi]. Jakarta: Universitas Pancasila.
[5]. Departemen Kesehatan Republik Indonesia. 1985. Cara Pembuatan Simplisia Jakarta: Direktorat Jendral Pengawasan Obat dan Makanan. Direktorat Pengawasan Obat Tradisional: 1-2.
[6]. Associati of Official Analytical Chemist. 2000. Official Method 942.05 Ash ofanimal feed. AOAC Official Method 4:6.
[7]. Associati of Official Analytical Chemist. 2000. Official Method 942.05 Ash ofanimal feed. AOAC Official Method 4:6.
[8]. Wahyuni S. 2010. Karakterisasi Senyawa Bioaktif Isoflavon dan Uji Aktivitas Antioksidan dari Ekstrak Tempe Berbahan Baku Buncis dan Kecipir. Surakarta : Program Pasca Sarjana Biosains Universitas Sebelas Maret.
[9]. Wahyuni S. 2010. Karakterisasi Senyawa Bioaktif Isoflavon dan Uji Aktivitas Antioksidan dari Ekstrak Tempe Berbahan Baku Buncis dan Kecipir. Surakarta : Program Pasca Sarjana Biosains Universitas Sebelas Maret.
[10]. Yuhernita dan Juniarti. 2011. Analisis Senyawa Metabolit Sekunder dari Ekstrak Metanol Daun Surian yang Berpotensi Sebagai Antioksidan. Makara, Sains. Vol. 15: 48-52.
[11]. Molyneux, P. 2004. The Use of The Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol. 26(2): 211-216
[12]. Hidajat, B. 2005. Penggunaan antioksidan pada anak. Surabaya: Fakultas Kedokteran Universitas Airlangga.
Published
2019-06-12
How to Cite
Eem Masaenah, Anna Priangani Roswiem, & Devina Putri. (2019). AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL 70% DAN INFUSA DAUN KECIPIR (Psophocarpus tetragonolobus (L.) DC.) DENGAN METODE PERENDAMAN RADIKAL BEBAS. Jurnal Farmamedika (Pharmamedika Journal), 4(1), 11-17. https://doi.org/10.47219/ath.v4i1.53